![]() coli cultures Up to 350g of plasmid in less than 30 min Compatible with vacuum and spin formats. Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure " Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription. Kit contents: Qiagen Plasmid Midi Kit, 25 preps, 25 to 100mL Culture Volume, Manual (Centrifugation) Processing, 150 min. Cat No./ID: 12143 QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers 379.00 Add To Cart Cat No. Plasmid DNA Midi Kit Isolate up to 200 g plasmid DNA from 15-50 mL bacterial cultures using midi spin columns Size REQUEST A SAMPLE 2 preps 25 preps 100 preps 184. The QIAGEN Plasmid Plus Kits provide transfection-grade plasmid DNA highly suited for all applications, such as: Transfection into most cell lines (e.g. Purify plasmid DNA with the GenElute HP plasmid midiprep kit Great for plasmid preparation from E. 19046) can be stored at room temperature (1525C) for up to 2 years if not otherwise stated on label. ( b) First the Level 0 parts will be released from the pUAP4 backbone via BsaI mediated digestion revealing the necessary part overhangs for Level 1 assembly. 12162, 1215) and the Plasmid Buffer Set (cat. Up toġ0 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). ( a) The plasmids containing the Level 0 parts are placed in the same tube with the pCk3chlo plasmid, the BsaI and the T4 ligase enzymes. The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. See more information regarding principle below.Performance 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. 12181) and the QIAGEN Plasmid Giga Kit (cat. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium. ![]() Up to 100 g high-copy plasmid DNA is purified from 25100 ml culture. cells were washed using PBS and cellular RNA was extracted using the RNAeasy 411 kit from Qiagen. Lysate clearing and isopropanol precipitation are achieved by centrifugation.The QIAGEN Plasmid Mega Kit (cat. Classic QIAGEN Plasmid Midi Kits use gravity-flow QIAGEN-tip 100 anion-exchange tips for efficient purification of plasmid DNA. Here, we describe the development of the CYL-02 31 non-viral gene-therapy product, that comprises a DNA-plasmid encoding for the three 32 aforementioned genes which expression is targeted to tumor cells. This kit can provide 100 preps, recovering up to 200 g of high copy plasmid DNA per prep. LyseBlue for optimum lysis and maximum DNA yieldQIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Kit contents: Qiagen Plasmid Midi Kit, 25 preps, 25 to 100mL Culture Volume, Manual (Centrifugation) Processing, 150 min. Plasmid DNA Midiprep Kits Deliver high-quality plasmid DNA up to 200 g with midiprep kits The GeneJET Plasmid Midiprep Kit is designed for large-scale isolation of plasmid DNA from recombinant E. Purity equivalent to 2 x CsCl gradient centrifugation įull paper Login or join for free to view the full paper.For purification of up to 10 mg transfection-grade plasmid or cosmid DNAFeatures All the plasmid vectors were purified with Qiagen Plasmid Plus Midi Kits (Qiagen K.K., Tokyo, Japan) and linearized with I-SceI (New England Biolabs, Ipswich, MA, USA) prior to transfection. Genomic fragments for homology arms were PCR amplified with attB containing primers (HPRT-SH 5′Fw and HPRT 5′Rv for the 5′ arm and HPRT 3′Fw and HPRT 3′Rv for the 3′ arm). Similarly, another HPRT targeting vector, pHPRT-SH 2A-EGFP-2A-Puro, was constructed using the MultiSite Gateway system to assemble two homology arms (1.7 and 2.8-kb) and a drug-resistance gene cassette. ![]() By using the MultiSite Gateway system (Life Technologies, Rockville, MD, USA), a floxed Hyg R or Puro R gene was placed between the 5′ and 3′ arms, thus yielding targeting vectors pHPRT-LH IRES2-Hyg and pHPRT-LH 2A-EGFP-2A-Puro. To generate targeting vectors for the human HPRT gene, 3.8 and 2.8-kb HPRT genomic fragments were PCR amplified with attB containing primers (HPRT-LH 5′Fw and HPRT 5′Rv for the 3.8-kb 5′ arm and HPRT 3′Fw and HPRT 3′Rv for the 2.8-kb 3′ arm). ![]()
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